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-rw-r--r--gnu/packages/bioinformatics.scm1133
1 files changed, 1051 insertions, 82 deletions
diff --git a/gnu/packages/bioinformatics.scm b/gnu/packages/bioinformatics.scm
index b758c72dec..d0873c9676 100644
--- a/gnu/packages/bioinformatics.scm
+++ b/gnu/packages/bioinformatics.scm
@@ -110,32 +110,6 @@
#:use-module (srfi srfi-1)
#:use-module (ice-9 match))
-(define-public r-ape
- (package
- (name "r-ape")
- (version "5.0")
- (source
- (origin
- (method url-fetch)
- (uri (cran-uri "ape" version))
- (sha256
- (base32
- "0q59pmxawz498cb9mv5m49lhiwxib8ak94yyydz7qg8b6lpd4bn3"))))
- (build-system r-build-system)
- (propagated-inputs
- `(("r-lattice" ,r-lattice)
- ("r-nlme" ,r-nlme)
- ("r-rcpp" ,r-rcpp)))
- (home-page "http://ape-package.ird.fr/")
- (synopsis "Analyses of phylogenetics and evolution")
- (description
- "This package provides functions for reading, writing, plotting, and
-manipulating phylogenetic trees, analyses of comparative data in a
-phylogenetic framework, ancestral character analyses, analyses of
-diversification and macroevolution, computing distances from DNA sequences,
-and several other tools.")
- (license license:gpl2+)))
-
(define-public aragorn
(package
(name "aragorn")
@@ -1854,38 +1828,22 @@ file formats including SAM/BAM, Wiggle/BigWig, BED, GFF/GTF, VCF.")
(define-public cutadapt
(package
(name "cutadapt")
- (version "1.14")
+ (version "1.16")
(source (origin
- (method url-fetch)
- (uri (string-append
- "https://github.com/marcelm/cutadapt/archive/v"
- version ".tar.gz"))
- (file-name (string-append name "-" version ".tar.gz"))
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/marcelm/cutadapt.git")
+ (commit (string-append "v" version))))
+ (file-name (string-append name "-" version "-checkout"))
(sha256
(base32
- "16gbpiwy4m48vq2h5wqar3i8vr6vcj9gcl2qvqim19x6ya9dp8kd"))))
+ "09pr02067jiks19nc0aby4xp70hhgvb554i2y1c04rv1m401w7q8"))))
(build-system python-build-system)
- (arguments
- `(#:phases
- (modify-phases %standard-phases
- ;; The tests must be run after installation.
- (delete 'check)
- (add-after 'install 'check
- (lambda* (#:key inputs outputs #:allow-other-keys)
- (setenv "PYTHONPATH"
- (string-append
- (getenv "PYTHONPATH")
- ":" (assoc-ref outputs "out")
- "/lib/python"
- (string-take (string-take-right
- (assoc-ref inputs "python") 5) 3)
- "/site-packages"))
- (zero? (system* "nosetests" "-P" "tests")))))))
(inputs
`(("python-xopen" ,python-xopen)))
(native-inputs
`(("python-cython" ,python-cython)
- ("python-nose" ,python-nose)))
+ ("python-pytest" ,python-pytest)))
(home-page "https://cutadapt.readthedocs.io/en/stable/")
(synopsis "Remove adapter sequences from nucleotide sequencing reads")
(description
@@ -3019,6 +2977,76 @@ sequencing (HTS) data. There are also an number of useful utilities for
manipulating HTS data.")
(license license:expat)))
+(define-public java-htsjdk-latest
+ (package
+ (name "java-htsjdk")
+ (version "2.14.3")
+ (source (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/samtools/htsjdk.git")
+ (commit version)))
+ (file-name (string-append name "-" version "-checkout"))
+ (sha256
+ (base32
+ "1lmya1fdjy03mz6zmdmd86j9v9vfhqb3952mqq075navx1i6g4bc"))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:tests? #f ; test require Scala
+ #:jdk ,icedtea-8
+ #:jar-name "htsjdk.jar"
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'remove-useless-build.xml
+ (lambda _ (delete-file "build.xml") #t))
+ ;; The tests require the scalatest package.
+ (add-after 'unpack 'remove-tests
+ (lambda _ (delete-file-recursively "src/test") #t)))))
+ (inputs
+ `(("java-ngs" ,java-ngs)
+ ("java-snappy-1" ,java-snappy-1)
+ ("java-commons-compress" ,java-commons-compress)
+ ("java-commons-logging-minimal" ,java-commons-logging-minimal)
+ ("java-commons-jexl-2" ,java-commons-jexl-2)
+ ("java-xz" ,java-xz)))
+ (native-inputs
+ `(("java-junit" ,java-junit)))
+ (home-page "http://samtools.github.io/htsjdk/")
+ (synopsis "Java API for high-throughput sequencing data (HTS) formats")
+ (description
+ "HTSJDK is an implementation of a unified Java library for accessing
+common file formats, such as SAM and VCF, used for high-throughput
+sequencing (HTS) data. There are also an number of useful utilities for
+manipulating HTS data.")
+ (license license:expat)))
+
+;; This is needed for picard 2.10.3
+(define-public java-htsjdk-2.10.1
+ (package (inherit java-htsjdk-latest)
+ (name "java-htsjdk")
+ (version "2.10.1")
+ (source (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/samtools/htsjdk.git")
+ (commit version)))
+ (file-name (string-append name "-" version "-checkout"))
+ (sha256
+ (base32
+ "1kxh7slm2pm3x9p6jxa1wqsq9a31dhiiflhxnxqcisan4k3rwia2"))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:tests? #f ; tests require Scala
+ #:jdk ,icedtea-8
+ #:jar-name "htsjdk.jar"
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'remove-useless-build.xml
+ (lambda _ (delete-file "build.xml") #t))
+ ;; The tests require the scalatest package.
+ (add-after 'unpack 'remove-tests
+ (lambda _ (delete-file-recursively "src/test") #t)))))))
+
;; This version matches java-htsjdk 2.3.0. Later versions also require a more
;; recent version of java-htsjdk, which depends on gradle.
(define-public java-picard
@@ -3097,6 +3125,90 @@ commonly used for high-throughput sequencing data such as SAM, BAM, CRAM and
VCF.")
(license license:expat)))
+;; This is needed for dropseq-tools
+(define-public java-picard-2.10.3
+ (package
+ (name "java-picard")
+ (version "2.10.3")
+ (source (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/broadinstitute/picard.git")
+ (commit version)))
+ (file-name (string-append "java-picard-" version "-checkout"))
+ (sha256
+ (base32
+ "1ajlx31l6i1k3y2rhnmgq07sz99g2czqfqgkr9mihmdjp3gwjhvi"))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:jar-name "picard.jar"
+ ;; Tests require jacoco:coverage.
+ #:tests? #f
+ #:jdk ,icedtea-8
+ #:main-class "picard.cmdline.PicardCommandLine"
+ #:modules ((guix build ant-build-system)
+ (guix build utils)
+ (guix build java-utils)
+ (sxml simple)
+ (sxml transform)
+ (sxml xpath))
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'remove-useless-build.xml
+ (lambda _ (delete-file "build.xml") #t))
+ ;; This is necessary to ensure that htsjdk is found when using
+ ;; picard.jar as an executable.
+ (add-before 'build 'edit-classpath-in-manifest
+ (lambda* (#:key inputs #:allow-other-keys)
+ (chmod "build.xml" #o664)
+ (call-with-output-file "build.xml.new"
+ (lambda (port)
+ (sxml->xml
+ (pre-post-order
+ (with-input-from-file "build.xml"
+ (lambda _ (xml->sxml #:trim-whitespace? #t)))
+ `((target . ,(lambda (tag . kids)
+ (let ((name ((sxpath '(name *text*))
+ (car kids)))
+ ;; FIXME: We're breaking the line
+ ;; early with a dummy path to
+ ;; ensure that the store reference
+ ;; isn't broken apart and can still
+ ;; be found by the reference
+ ;; scanner.
+ (msg (format #f
+ "\
+Class-Path: /~a \
+ ~a/share/java/htsjdk.jar${line.separator}"
+ ;; maximum line length is 70
+ (string-tabulate (const #\b) 57)
+ (assoc-ref inputs "java-htsjdk"))))
+ (if (member "manifest" name)
+ `(,tag ,@kids
+ (echo
+ (@ (message ,msg)
+ (file "${manifest.file}")
+ (append "true"))))
+ `(,tag ,@kids)))))
+ (*default* . ,(lambda (tag . kids) `(,tag ,@kids)))
+ (*text* . ,(lambda (_ txt) txt))))
+ port)))
+ (rename-file "build.xml.new" "build.xml")
+ #t)))))
+ (propagated-inputs
+ `(("java-htsjdk" ,java-htsjdk-2.10.1)))
+ (native-inputs
+ `(("java-testng" ,java-testng)
+ ("java-guava" ,java-guava)))
+ (home-page "http://broadinstitute.github.io/picard/")
+ (synopsis "Tools for manipulating high-throughput sequencing data and formats")
+ (description "Picard is a set of Java command line tools for manipulating
+high-throughput sequencing (HTS) data and formats. Picard is implemented
+using the HTSJDK Java library to support accessing file formats that are
+commonly used for high-throughput sequencing data such as SAM, BAM, CRAM and
+VCF.")
+ (license license:expat)))
+
;; This is the last version of Picard to provide net.sf.samtools
(define-public java-picard-1.113
(package (inherit java-picard)
@@ -3211,10 +3323,89 @@ VCF.")
("jdk" ,icedtea-8 "jdk")
("jdk-src" ,(car (assoc-ref (package-native-inputs icedtea-8) "jdk-drop")))))))
+(define-public fastqc
+ (package
+ (name "fastqc")
+ (version "0.11.5")
+ (source
+ (origin
+ (method url-fetch)
+ (uri (string-append "http://www.bioinformatics.babraham.ac.uk/"
+ "projects/fastqc/fastqc_v"
+ version "_source.zip"))
+ (sha256
+ (base32
+ "18rrlkhcrxvvvlapch4dpj6xc6mpayzys8qfppybi8jrpgx5cc5f"))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:tests? #f ; there are no tests
+ #:build-target "build"
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'fix-dependencies
+ (lambda* (#:key inputs #:allow-other-keys)
+ (substitute* "build.xml"
+ (("jbzip2-0.9.jar")
+ (string-append (assoc-ref inputs "java-jbzip2")
+ "/share/java/jbzip2.jar"))
+ (("sam-1.103.jar")
+ (string-append (assoc-ref inputs "java-picard-1.113")
+ "/share/java/sam-1.112.jar"))
+ (("cisd-jhdf5.jar")
+ (string-append (assoc-ref inputs "java-cisd-jhdf5")
+ "/share/java/sis-jhdf5.jar")))
+ #t))
+ ;; There is no installation target
+ (replace 'install
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let* ((out (assoc-ref outputs "out"))
+ (bin (string-append out "/bin"))
+ (share (string-append out "/share/fastqc/"))
+ (exe (string-append share "/fastqc")))
+ (for-each mkdir-p (list bin share))
+ (copy-recursively "bin" share)
+ (substitute* exe
+ (("my \\$java_bin = 'java';")
+ (string-append "my $java_bin = '"
+ (assoc-ref inputs "java")
+ "/bin/java';")))
+ (chmod exe #o555)
+ (symlink exe (string-append bin "/fastqc"))
+ #t))))))
+ (inputs
+ `(("java" ,icedtea)
+ ("perl" ,perl) ; needed for the wrapper script
+ ("java-cisd-jhdf5" ,java-cisd-jhdf5)
+ ("java-picard-1.113" ,java-picard-1.113)
+ ("java-jbzip2" ,java-jbzip2)))
+ (native-inputs
+ `(("unzip" ,unzip)))
+ (home-page "http://www.bioinformatics.babraham.ac.uk/projects/fastqc/")
+ (synopsis "Quality control tool for high throughput sequence data")
+ (description
+ "FastQC aims to provide a simple way to do some quality control
+checks on raw sequence data coming from high throughput sequencing
+pipelines. It provides a modular set of analyses which you can use to
+give a quick impression of whether your data has any problems of which
+you should be aware before doing any further analysis.
+
+The main functions of FastQC are:
+
+@itemize
+@item Import of data from BAM, SAM or FastQ files (any variant);
+@item Providing a quick overview to tell you in which areas there may
+ be problems;
+@item Summary graphs and tables to quickly assess your data;
+@item Export of results to an HTML based permanent report;
+@item Offline operation to allow automated generation of reports
+ without running the interactive application.
+@end itemize\n")
+ (license license:gpl3+)))
+
(define-public htslib
(package
(name "htslib")
- (version "1.6")
+ (version "1.7")
(source (origin
(method url-fetch)
(uri (string-append
@@ -3222,17 +3413,8 @@ VCF.")
version "/htslib-" version ".tar.bz2"))
(sha256
(base32
- "1jsca3hg4rbr6iqq6imkj4lsvgl8g9768bcmny3hlff2w25vx24m"))))
+ "1il6i2p84b0y9c93dhvzzki1ifw9bvapm2mvpr0xvb2nq8jlwgdy"))))
(build-system gnu-build-system)
- (arguments
- `(#:phases
- (modify-phases %standard-phases
- (add-after
- 'unpack 'patch-tests
- (lambda _
- (substitute* "test/test.pl"
- (("/bin/bash") (which "bash")))
- #t)))))
(inputs
`(("openssl" ,openssl)
("curl" ,curl)
@@ -4629,6 +4811,7 @@ distribution, coverage uniformity, strand specificity, etc.")
(uri (hg-reference
(url "https://bitbucket.org/libsleipnir/sleipnir")
(changeset changeset)))
+ (file-name (string-append name "-" version "-checkout"))
(sha256
(base32
"0qrvilwh18dpbhkf92qvxbmay0j75ra3jg2wrhz67gf538zzphsx"))))
@@ -4687,7 +4870,7 @@ to the user's query of interest.")
(define-public samtools
(package
(name "samtools")
- (version "1.5")
+ (version "1.7")
(source
(origin
(method url-fetch)
@@ -4696,7 +4879,7 @@ to the user's query of interest.")
version "/samtools-" version ".tar.bz2"))
(sha256
(base32
- "1xidmv0jmfy7l0kb32hdnlshcxgzi1hmygvig0cqrq1fhckdlhl5"))))
+ "18acyqysbxpydlc44lqv2hpp57l06bs9a3yqmcvjk8va2xrrdc77"))))
(build-system gnu-build-system)
(arguments
`(#:modules ((ice-9 ftw)
@@ -6017,14 +6200,14 @@ distribution.")
(define-public r-dexseq
(package
(name "r-dexseq")
- (version "1.24.2")
+ (version "1.24.3")
(source
(origin
(method url-fetch)
(uri (bioconductor-uri "DEXSeq" version))
(sha256
(base32
- "18nh8ynxirfwkmc4sawdxgl7w1sl9ny5zpv8zbhv9vi5vgb8pxmj"))))
+ "0xip73hlbr3zry9d7ly9vvvsbb3xjcmfa09lr9fdy528dwjrf084"))))
(properties `((upstream-name . "DEXSeq")))
(build-system r-build-system)
(propagated-inputs
@@ -6200,14 +6383,14 @@ testing and other simple calculations.")
(define-public r-shortread
(package
(name "r-shortread")
- (version "1.36.0")
+ (version "1.36.1")
(source
(origin
(method url-fetch)
(uri (bioconductor-uri "ShortRead" version))
(sha256
(base32
- "06mknlsmd4hnaxzdjapgvp2kgdnf9w103y500dsac5jgsz4vwzcz"))))
+ "1cyv47632m9ljkxfsvnvmd19sb607ys5kz8fwh6v39dnw16g0a6m"))))
(properties `((upstream-name . "ShortRead")))
(build-system r-build-system)
(inputs
@@ -6968,13 +7151,13 @@ names in their natural, rather than lexicographic, order.")
(define-public r-edger
(package
(name "r-edger")
- (version "3.20.8")
+ (version "3.20.9")
(source (origin
(method url-fetch)
(uri (bioconductor-uri "edgeR" version))
(sha256
(base32
- "0g7bj6w61blw3m22hw9rc01n554k9qkjizh7njr3j4shmhads58d"))))
+ "0y52snwbz37xzdd7gihdkqczbndlfzmmypv6hri3ymjyfmlx6qaw"))))
(properties `((upstream-name . "edgeR")))
(build-system r-build-system)
(propagated-inputs
@@ -7034,13 +7217,13 @@ coding changes and predict coding outcomes.")
(define-public r-limma
(package
(name "r-limma")
- (version "3.34.8")
+ (version "3.34.9")
(source (origin
(method url-fetch)
(uri (bioconductor-uri "limma" version))
(sha256
(base32
- "0bmxsgz2yll83sd3wbxsrsfd35468igb0d8lldym0d0lqfz906bw"))))
+ "1y2fm61g5i0fn0j3l31xvwh9zww9bpkc4nwzb1d0yv1cag20jkdc"))))
(build-system r-build-system)
(home-page "http://bioinf.wehi.edu.au/limma")
(synopsis "Package for linear models for microarray and RNA-seq data")
@@ -7089,13 +7272,13 @@ different technologies, including microarrays, RNA-seq, and quantitative PCR.")
(define-public r-genomicranges
(package
(name "r-genomicranges")
- (version "1.30.2")
+ (version "1.30.3")
(source (origin
(method url-fetch)
(uri (bioconductor-uri "GenomicRanges" version))
(sha256
(base32
- "0c3r155603vb4zjs3adqa72770bh8karc11y8gl62l1m24jrkbnj"))))
+ "07cszc9ri94nzk4dffwnsj247ih6pchnrzrvnb0q4dkk33gwy8n1"))))
(properties
`((upstream-name . "GenomicRanges")))
(build-system r-build-system)
@@ -7519,13 +7702,13 @@ information about the latest version of the Gene Ontologies.")
(define-public r-topgo
(package
(name "r-topgo")
- (version "2.30.0")
+ (version "2.30.1")
(source (origin
(method url-fetch)
(uri (bioconductor-uri "topGO" version))
(sha256
(base32
- "1hqffz5qp7glxdvjp37005g8qk5nam3f9wpf6d1wjnzpar04f3dz"))))
+ "1cgz4knxr328xfqlhl6ypxl6x86rfrlqz748kn94ainxjzz55i6x"))))
(properties
`((upstream-name . "topGO")))
(build-system r-build-system)
@@ -8365,14 +8548,14 @@ in SNV base substitution data.")
(define-public r-wgcna
(package
(name "r-wgcna")
- (version "1.62")
+ (version "1.63")
(source
(origin
(method url-fetch)
(uri (cran-uri "WGCNA" version))
(sha256
(base32
- "0c52rp09gqphz6g5x9jzkdcsyvfrknq0dkq9saslgy8q8ap974vx"))))
+ "1225dqm68bynkmklnsxdqdd3zqrpzbvqwyly8ibxmk75z33xz309"))))
(properties `((upstream-name . "WGCNA")))
(build-system r-build-system)
(propagated-inputs
@@ -8542,6 +8725,16 @@ of gene-level counts.")
"src/hdf5source/hdf5small.tgz" "-C" "src/" )
(substitute* "src/hdf5/configure"
(("/bin/mv") "mv"))
+ ;; Remove timestamp and host system information to make
+ ;; the build reproducible.
+ (substitute* "src/hdf5/src/libhdf5.settings.in"
+ (("Configured on: @CONFIG_DATE@")
+ "Configured on: Guix")
+ (("Uname information:.*")
+ "Uname information: Linux\n")
+ ;; Remove unnecessary store reference.
+ (("C Compiler:.*")
+ "C Compiler: GCC\n"))
#t)))))
(propagated-inputs
`(("r-zlibbioc" ,r-zlibbioc)))
@@ -8706,6 +8899,7 @@ intervals (e.g. genes, sequence alignments).")
(uri (git-reference
(url "https://github.com/smithlabcode/piranha.git")
(commit commit)))
+ (file-name (git-file-name name version))
(sha256
(base32
"117dc0zf20c61jam69sk4abl57ah6yi6i7qra7d7y5zrbgk12q5n"))))
@@ -10460,6 +10654,16 @@ block processing.")
(invoke "tar" "xvf" (assoc-ref inputs "hdf5-source"))
(rename-file (string-append "hdf5-" ,(package-version hdf5))
"hdf5")
+ ;; Remove timestamp and host system information to make
+ ;; the build reproducible.
+ (substitute* "hdf5/src/libhdf5.settings.in"
+ (("Configured on: @CONFIG_DATE@")
+ "Configured on: Guix")
+ (("Uname information:.*")
+ "Uname information: Linux\n")
+ ;; Remove unnecessary store reference.
+ (("C Compiler:.*")
+ "C Compiler: GCC\n"))
(rename-file "Makevars.in" "Makevars")
(substitute* "Makevars"
(("HDF5_CXX_LIB=.*")
@@ -10588,14 +10792,14 @@ quality control.")
(define-public r-scran
(package
(name "r-scran")
- (version "1.6.7")
+ (version "1.6.8")
(source
(origin
(method url-fetch)
(uri (bioconductor-uri "scran" version))
(sha256
(base32
- "0zfm5i8xrnzb3894xygqd6zkbwaa2x3z74wbxw39fcjhyhxv2hmb"))))
+ "07wniyrh2fhhkz28v0bfgpvpi1hkkn2cvhacrvvvck142j79944x"))))
(build-system r-build-system)
(propagated-inputs
`(("r-beachmat" ,r-beachmat)
@@ -10659,9 +10863,37 @@ are optimized per data type and for subsetted calculations such that both
memory usage and processing time is minimized.")
(license license:expat)))
+(define-public r-phangorn
+ (package
+ (name "r-phangorn")
+ (version "2.4.0")
+ (source
+ (origin
+ (method url-fetch)
+ (uri (cran-uri "phangorn" version))
+ (sha256
+ (base32
+ "0xc8k552nxczy19jr0xjjagrzc8x6lafasgk2c099ls8bc1yml1i"))))
+ (build-system r-build-system)
+ (propagated-inputs
+ `(("r-ape" ,r-ape)
+ ("r-fastmatch" ,r-fastmatch)
+ ("r-igraph" ,r-igraph)
+ ("r-magrittr" ,r-magrittr)
+ ("r-matrix" ,r-matrix)
+ ("r-quadprog" ,r-quadprog)
+ ("r-rcpp" ,r-rcpp)))
+ (home-page "https://github.com/KlausVigo/phangorn")
+ (synopsis "Phylogenetic analysis in R")
+ (description
+ "Phangorn is a package for phylogenetic analysis in R. It supports
+estimation of phylogenetic trees and networks using Maximum Likelihood,
+Maximum Parsimony, distance methods and Hadamard conjugation.")
+ (license license:gpl2+)))
+
(define-public r-dropbead
- (let ((commit "cf0be5ae5302684bd03e78ab65b142900bbbb840")
- (revision "1"))
+ (let ((commit "d746c6f3b32110428ea56d6a0001ce52a251c247")
+ (revision "2"))
(package
(name "r-dropbead")
(version (string-append "0-" revision "." (string-take commit 7)))
@@ -10671,9 +10903,10 @@ memory usage and processing time is minimized.")
(uri (git-reference
(url "https://github.com/rajewsky-lab/dropbead.git")
(commit commit)))
+ (file-name (git-file-name name version))
(sha256
(base32
- "1b2lphsc236s1rdzlijxg8yl1jnqpwcvj4x938r89rqpj93jb780"))))
+ "0sbzma49aiiyw8b0jpr7fnhzys9nsqmp4hy4hdz1gzyg1lhnca26"))))
(build-system r-build-system)
(propagated-inputs
`(("r-ggplot2" ,r-ggplot2)
@@ -11424,6 +11657,7 @@ dependency like SeqAn.")
(uri (git-reference
(url "https://github.com/y-256/libdivsufsort.git")
(commit version)))
+ (file-name (git-file-name name version))
(sha256
(base32
"0fgdz9fzihlvjjrxy01md1bv9vh12rkgkwbm90b1hj5xpbaqp7z2"))))
@@ -11879,3 +12113,738 @@ variable number of row and column annotations. Loom also supports sparse
graphs. This library makes it easy to work with @file{.loom} files for
single-cell RNA-seq data.")
(license license:bsd-3)))
+
+;; We cannot use the latest commit because it requires Java 9.
+(define-public java-forester
+ (let ((commit "86b07efe302d5094b42deed9260f719a4c4ac2e6")
+ (revision "1"))
+ (package
+ (name "java-forester")
+ (version (string-append "0-" revision "." (string-take commit 7)))
+ (source (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/cmzmasek/forester.git")
+ (commit commit)))
+ (file-name (string-append name "-" version "-checkout"))
+ (sha256
+ (base32
+ "0vxavc1yrf84yrnf20dq26hi0lglidk8d382xrxsy4qmlbjd276z"))
+ (modules '((guix build utils)))
+ (snippet
+ '(begin
+ ;; Delete bundled jars and pre-built classes
+ (delete-file-recursively "forester/java/resources")
+ (delete-file-recursively "forester/java/classes")
+ (for-each delete-file (find-files "forester/java/" "\\.jar$"))
+ ;; Delete bundled applications
+ (delete-file-recursively "forester_applications")
+ #t))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:tests? #f ; there are none
+ #:jdk ,icedtea-8
+ #:modules ((guix build ant-build-system)
+ (guix build utils)
+ (guix build java-utils)
+ (sxml simple)
+ (sxml transform))
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'chdir
+ (lambda _ (chdir "forester/java") #t))
+ (add-after 'chdir 'fix-dependencies
+ (lambda _
+ (chmod "build.xml" #o664)
+ (call-with-output-file "build.xml.new"
+ (lambda (port)
+ (sxml->xml
+ (pre-post-order
+ (with-input-from-file "build.xml"
+ (lambda _ (xml->sxml #:trim-whitespace? #t)))
+ `(;; Remove all unjar tags to avoid repacking classes.
+ (unjar . ,(lambda _ '()))
+ (*default* . ,(lambda (tag . kids) `(,tag ,@kids)))
+ (*text* . ,(lambda (_ txt) txt))))
+ port)))
+ (rename-file "build.xml.new" "build.xml")
+ #t))
+ ;; FIXME: itext is difficult to package as it depends on a few
+ ;; unpackaged libraries.
+ (add-after 'chdir 'remove-dependency-on-unpackaged-itext
+ (lambda _
+ (delete-file "src/org/forester/archaeopteryx/PdfExporter.java")
+ (substitute* "src/org/forester/archaeopteryx/MainFrame.java"
+ (("pdf_written_to = PdfExporter.*")
+ "throw new IOException(\"PDF export is not available.\");"))
+ #t))
+ ;; There is no install target
+ (replace 'install (install-jars ".")))))
+ (propagated-inputs
+ `(("java-commons-codec" ,java-commons-codec)
+ ("java-openchart2" ,java-openchart2)))
+ (home-page "https://sites.google.com/site/cmzmasek/home/software/forester")
+ (synopsis "Phylogenomics libraries for Java")
+ (description "Forester is a collection of Java libraries for
+phylogenomics and evolutionary biology research. It includes support for
+reading, writing, and exporting phylogenetic trees.")
+ (license license:lgpl2.1+))))
+
+(define-public java-forester-1.005
+ (package
+ (name "java-forester")
+ (version "1.005")
+ (source (origin
+ (method url-fetch)
+ (uri (string-append "http://search.maven.org/remotecontent?"
+ "filepath=org/biojava/thirdparty/forester/"
+ version "/forester-" version "-sources.jar"))
+ (file-name (string-append name "-" version ".jar"))
+ (sha256
+ (base32
+ "04r8qv4rk3p71z4ajrvp11py1z46qrx0047j3zzs79s6lnsm3lcv"))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:tests? #f ; there are none
+ #:jdk ,icedtea-8
+ #:modules ((guix build ant-build-system)
+ (guix build utils)
+ (guix build java-utils)
+ (sxml simple)
+ (sxml transform))
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'unpack 'fix-dependencies
+ (lambda* (#:key inputs #:allow-other-keys)
+ (call-with-output-file "build.xml"
+ (lambda (port)
+ (sxml->xml
+ (pre-post-order
+ (with-input-from-file "src/build.xml"
+ (lambda _ (xml->sxml #:trim-whitespace? #t)))
+ `(;; Remove all unjar tags to avoid repacking classes.
+ (unjar . ,(lambda _ '()))
+ (*default* . ,(lambda (tag . kids) `(,tag ,@kids)))
+ (*text* . ,(lambda (_ txt) txt))))
+ port)))
+ (copy-file (assoc-ref inputs "synth_look_and_feel_1.xml")
+ "synth_look_and_feel_1.xml")
+ (copy-file (assoc-ref inputs "phyloxml.xsd")
+ "phyloxml.xsd")
+ (substitute* "build.xml"
+ (("../resources/synth_laf/synth_look_and_feel_1.xml")
+ "synth_look_and_feel_1.xml")
+ (("../resources/phyloxml_schema/1.10/phyloxml.xsd")
+ "phyloxml.xsd"))
+ #t))
+ ;; FIXME: itext is difficult to package as it depends on a few
+ ;; unpackaged libraries.
+ (add-after 'unpack 'remove-dependency-on-unpackaged-itext
+ (lambda _
+ (delete-file "src/org/forester/archaeopteryx/PdfExporter.java")
+ (substitute* '("src/org/forester/archaeopteryx/MainFrame.java"
+ "src/org/forester/archaeopteryx/MainFrameApplication.java")
+ (("pdf_written_to = PdfExporter.*")
+ "throw new IOException(\"PDF export is not available.\"); /*")
+ ((".getPrintSizeX\\(\\), getOptions\\(\\).getPrintSizeY\\(\\) \\);") "*/")
+ (("getCurrentTreePanel\\(\\).getHeight\\(\\) \\);") "*/"))
+ #t))
+ (add-after 'unpack 'delete-pre-built-classes
+ (lambda _ (delete-file-recursively "src/classes") #t))
+ ;; There is no install target
+ (replace 'install (install-jars ".")))))
+ (propagated-inputs
+ `(("java-commons-codec" ,java-commons-codec)
+ ("java-openchart2" ,java-openchart2)))
+ ;; The source archive does not contain the resources.
+ (native-inputs
+ `(("phyloxml.xsd"
+ ,(origin
+ (method url-fetch)
+ (uri (string-append "https://raw.githubusercontent.com/cmzmasek/forester/"
+ "b61cc2dcede0bede317db362472333115756b8c6/"
+ "forester/resources/phyloxml_schema/1.10/phyloxml.xsd"))
+ (file-name (string-append name "-phyloxml-" version ".xsd"))
+ (sha256
+ (base32
+ "1zxc4m8sn4n389nqdnpxa8d0k17qnr3pm2y5y6g6vh4k0zm52npv"))))
+ ("synth_look_and_feel_1.xml"
+ ,(origin
+ (method url-fetch)
+ (uri (string-append "https://raw.githubusercontent.com/cmzmasek/forester/"
+ "29e04321615da6b35c1e15c60e52caf3f21d8e6a/"
+ "forester/java/classes/resources/synth_look_and_feel_1.xml"))
+ (file-name (string-append name "-synth-look-and-feel-" version ".xml"))
+ (sha256
+ (base32
+ "1gv5602gv4k7y7713y75a4jvj7i9s7nildsbdl7n9q10sc2ikg8h"))))))
+ (home-page "https://sites.google.com/site/cmzmasek/home/software/forester")
+ (synopsis "Phylogenomics libraries for Java")
+ (description "Forester is a collection of Java libraries for
+phylogenomics and evolutionary biology research. It includes support for
+reading, writing, and exporting phylogenetic trees.")
+ (license license:lgpl2.1+)))
+
+(define-public java-biojava-core
+ (package
+ (name "java-biojava-core")
+ (version "4.2.11")
+ (source (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/biojava/biojava")
+ (commit (string-append "biojava-" version))))
+ (file-name (string-append name "-" version "-checkout"))
+ (sha256
+ (base32
+ "1bvryh2bpsvash8ln79cmc9sqm8qw72hz4xzwqxcrjm8ssxszhqk"))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:jdk ,icedtea-8
+ #:jar-name "biojava-core.jar"
+ #:source-dir "biojava-core/src/main/java/"
+ #:test-dir "biojava-core/src/test"
+ ;; These tests seem to require internet access.
+ #:test-exclude (list "**/SearchIOTest.java"
+ "**/BlastXMLParserTest.java"
+ "**/GenbankCookbookTest.java"
+ "**/GenbankProxySequenceReaderTest.java")
+ #:phases
+ (modify-phases %standard-phases
+ (add-before 'build 'copy-resources
+ (lambda _
+ (copy-recursively "biojava-core/src/main/resources"
+ "build/classes")
+ #t))
+ (add-before 'check 'copy-test-resources
+ (lambda _
+ (copy-recursively "biojava-core/src/test/resources"
+ "build/test-classes")
+ #t)))))
+ (propagated-inputs
+ `(("java-log4j-api" ,java-log4j-api)
+ ("java-log4j-core" ,java-log4j-core)
+ ("java-slf4j-api" ,java-slf4j-api)
+ ("java-slf4j-simple" ,java-slf4j-simple)))
+ (native-inputs
+ `(("java-junit" ,java-junit)
+ ("java-hamcrest-core" ,java-hamcrest-core)))
+ (home-page "http://biojava.org")
+ (synopsis "Core libraries of Java framework for processing biological data")
+ (description "BioJava is a project dedicated to providing a Java framework
+for processing biological data. It provides analytical and statistical
+routines, parsers for common file formats, reference implementations of
+popular algorithms, and allows the manipulation of sequences and 3D
+structures. The goal of the biojava project is to facilitate rapid
+application development for bioinformatics.
+
+This package provides the core libraries.")
+ (license license:lgpl2.1+)))
+
+(define-public java-biojava-phylo
+ (package (inherit java-biojava-core)
+ (name "java-biojava-phylo")
+ (build-system ant-build-system)
+ (arguments
+ `(#:jdk ,icedtea-8
+ #:jar-name "biojava-phylo.jar"
+ #:source-dir "biojava-phylo/src/main/java/"
+ #:test-dir "biojava-phylo/src/test"
+ #:phases
+ (modify-phases %standard-phases
+ (add-before 'build 'copy-resources
+ (lambda _
+ (copy-recursively "biojava-phylo/src/main/resources"
+ "build/classes")
+ #t))
+ (add-before 'check 'copy-test-resources
+ (lambda _
+ (copy-recursively "biojava-phylo/src/test/resources"
+ "build/test-classes")
+ #t)))))
+ (propagated-inputs
+ `(("java-log4j-api" ,java-log4j-api)
+ ("java-log4j-core" ,java-log4j-core)
+ ("java-slf4j-api" ,java-slf4j-api)
+ ("java-slf4j-simple" ,java-slf4j-simple)
+ ("java-biojava-core" ,java-biojava-core)
+ ("java-forester" ,java-forester)))
+ (native-inputs
+ `(("java-junit" ,java-junit)
+ ("java-hamcrest-core" ,java-hamcrest-core)))
+ (home-page "http://biojava.org")
+ (synopsis "Biojava interface to the forester phylogenomics library")
+ (description "The phylo module provides a biojava interface layer to the
+forester phylogenomics library for constructing phylogenetic trees.")))
+
+(define-public java-biojava-alignment
+ (package (inherit java-biojava-core)
+ (name "java-biojava-alignment")
+ (build-system ant-build-system)
+ (arguments
+ `(#:jdk ,icedtea-8
+ #:jar-name "biojava-alignment.jar"
+ #:source-dir "biojava-alignment/src/main/java/"
+ #:test-dir "biojava-alignment/src/test"
+ #:phases
+ (modify-phases %standard-phases
+ (add-before 'build 'copy-resources
+ (lambda _
+ (copy-recursively "biojava-alignment/src/main/resources"
+ "build/classes")
+ #t))
+ (add-before 'check 'copy-test-resources
+ (lambda _
+ (copy-recursively "biojava-alignment/src/test/resources"
+ "build/test-classes")
+ #t)))))
+ (propagated-inputs
+ `(("java-log4j-api" ,java-log4j-api)
+ ("java-log4j-core" ,java-log4j-core)
+ ("java-slf4j-api" ,java-slf4j-api)
+ ("java-slf4j-simple" ,java-slf4j-simple)
+ ("java-biojava-core" ,java-biojava-core)
+ ("java-biojava-phylo" ,java-biojava-phylo)
+ ("java-forester" ,java-forester)))
+ (native-inputs
+ `(("java-junit" ,java-junit)
+ ("java-hamcrest-core" ,java-hamcrest-core)))
+ (home-page "http://biojava.org")
+ (synopsis "Biojava API for genetic sequence alignment")
+ (description "The alignment module of BioJava provides an API that
+contains
+
+@itemize
+@item implementations of dynamic programming algorithms for sequence
+ alignment;
+@item reading and writing of popular alignment file formats;
+@item a single-, or multi- threaded multiple sequence alignment algorithm.
+@end itemize\n")))
+
+(define-public java-biojava-core-4.0
+ (package (inherit java-biojava-core)
+ (name "java-biojava-core")
+ (version "4.0.0")
+ (source (origin
+ (method git-fetch)
+ (uri (git-reference
+ (url "https://github.com/biojava/biojava")
+ (commit (string-append "biojava-" version))))
+ (file-name (string-append name "-" version "-checkout"))
+ (sha256
+ (base32
+ "13675f6y9aqi7bi2lk3s1z7a22ynccjiqwa8izh7p97xi9wsfmd8"))))))
+
+(define-public java-biojava-phylo-4.0
+ (package (inherit java-biojava-core-4.0)
+ (name "java-biojava-phylo")
+ (build-system ant-build-system)
+ (arguments
+ `(#:jdk ,icedtea-8
+ #:jar-name "biojava-phylo.jar"
+ #:source-dir "biojava-phylo/src/main/java/"
+ #:test-dir "biojava-phylo/src/test"
+ #:phases
+ (modify-phases %standard-phases
+ (add-before 'build 'copy-resources
+ (lambda _
+ (copy-recursively "biojava-phylo/src/main/resources"
+ "build/classes")
+ #t))
+ (add-before 'check 'copy-test-resources
+ (lambda _
+ (copy-recursively "biojava-phylo/src/test/resources"
+ "build/test-classes")
+ #t)))))
+ (propagated-inputs
+ `(("java-log4j-api" ,java-log4j-api)
+ ("java-log4j-core" ,java-log4j-core)
+ ("java-slf4j-api" ,java-slf4j-api)
+ ("java-slf4j-simple" ,java-slf4j-simple)
+ ("java-biojava-core" ,java-biojava-core-4.0)
+ ("java-forester" ,java-forester-1.005)))
+ (native-inputs
+ `(("java-junit" ,java-junit)
+ ("java-hamcrest-core" ,java-hamcrest-core)))
+ (home-page "http://biojava.org")
+ (synopsis "Biojava interface to the forester phylogenomics library")
+ (description "The phylo module provides a biojava interface layer to the
+forester phylogenomics library for constructing phylogenetic trees.")))
+
+(define-public java-biojava-alignment-4.0
+ (package (inherit java-biojava-core-4.0)
+ (name "java-biojava-alignment")
+ (build-system ant-build-system)
+ (arguments
+ `(#:jdk ,icedtea-8
+ #:jar-name "biojava-alignment.jar"
+ #:source-dir "biojava-alignment/src/main/java/"
+ #:test-dir "biojava-alignment/src/test"
+ #:phases
+ (modify-phases %standard-phases
+ (add-before 'build 'copy-resources
+ (lambda _
+ (copy-recursively "biojava-alignment/src/main/resources"
+ "build/classes")
+ #t))
+ (add-before 'check 'copy-test-resources
+ (lambda _
+ (copy-recursively "biojava-alignment/src/test/resources"
+ "build/test-classes")
+ #t)))))
+ (propagated-inputs
+ `(("java-log4j-api" ,java-log4j-api)
+ ("java-log4j-core" ,java-log4j-core)
+ ("java-slf4j-api" ,java-slf4j-api)
+ ("java-slf4j-simple" ,java-slf4j-simple)
+ ("java-biojava-core" ,java-biojava-core-4.0)
+ ("java-biojava-phylo" ,java-biojava-phylo-4.0)
+ ("java-forester" ,java-forester-1.005)))
+ (native-inputs
+ `(("java-junit" ,java-junit)
+ ("java-hamcrest-core" ,java-hamcrest-core)))
+ (home-page "http://biojava.org")
+ (synopsis "Biojava API for genetic sequence alignment")
+ (description "The alignment module of BioJava provides an API that
+contains
+
+@itemize
+@item implementations of dynamic programming algorithms for sequence
+ alignment;
+@item reading and writing of popular alignment file formats;
+@item a single-, or multi- threaded multiple sequence alignment algorithm.
+@end itemize\n")))
+
+(define-public dropseq-tools
+ (package
+ (name "dropseq-tools")
+ (version "1.13")
+ (source
+ (origin
+ (method url-fetch)
+ (uri "http://mccarrolllab.com/download/1276/")
+ (file-name (string-append "dropseq-tools-" version ".zip"))
+ (sha256
+ (base32
+ "0yrffckxqk5l8b5xb6z4laq157zd9mdypr2p4b4vq2bhjzi1sj0s"))
+ ;; Delete bundled libraries
+ (modules '((guix build utils)))
+ (snippet
+ '(begin
+ (for-each delete-file (find-files "jar/lib" "\\.jar$"))
+ (delete-file-recursively "3rdParty")))))
+ (build-system ant-build-system)
+ (arguments
+ `(#:tests? #f ; test data are not included
+ #:test-target "test"
+ #:build-target "all"
+ #:source-dir "public/src/"
+ #:jdk ,icedtea-8
+ #:make-flags
+ (list (string-append "-Dpicard.executable.dir="
+ (assoc-ref %build-inputs "java-picard")
+ "/share/java/"))
+ #:modules ((ice-9 match)
+ (srfi srfi-1)
+ (guix build utils)
+ (guix build java-utils)
+ (guix build ant-build-system))
+ #:phases
+ (modify-phases %standard-phases
+ ;; All dependencies must be linked to "lib", because that's where
+ ;; they will be searched for when the Class-Path property of the
+ ;; manifest is computed.
+ (add-after 'unpack 'record-references
+ (lambda* (#:key inputs #:allow-other-keys)
+ (mkdir-p "jar/lib")
+ (let ((dirs (filter-map (match-lambda
+ ((name . dir)
+ (if (and (string-prefix? "java-" name)
+ (not (string=? name "java-testng")))
+ dir #f)))
+ inputs)))
+ (for-each (lambda (jar)
+ (symlink jar (string-append "jar/lib/" (basename jar))))
+ (append-map (lambda (dir) (find-files dir "\\.jar$"))
+ dirs)))
+ #t))
+ ;; There is no installation target
+ (replace 'install
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let* ((out (assoc-ref outputs "out"))
+ (bin (string-append out "/bin"))
+ (share (string-append out "/share/java/"))
+ (lib (string-append share "/lib/"))
+ (scripts (list "BAMTagHistogram"
+ "BAMTagofTagCounts"
+ "BaseDistributionAtReadPosition"
+ "CollapseBarcodesInPlace"
+ "CollapseTagWithContext"
+ "ConvertToRefFlat"
+ "CreateIntervalsFiles"
+ "DetectBeadSynthesisErrors"
+ "DigitalExpression"
+ "Drop-seq_alignment.sh"
+ "FilterBAM"
+ "FilterBAMByTag"
+ "GatherGeneGCLength"
+ "GatherMolecularBarcodeDistributionByGene"
+ "GatherReadQualityMetrics"
+ "PolyATrimmer"
+ "ReduceGTF"
+ "SelectCellsByNumTranscripts"
+ "SingleCellRnaSeqMetricsCollector"
+ "TagBamWithReadSequenceExtended"
+ "TagReadWithGeneExon"
+ "TagReadWithInterval"
+ "TrimStartingSequence"
+ "ValidateReference")))
+ (for-each mkdir-p (list bin share lib))
+ (install-file "dist/dropseq.jar" share)
+ (for-each (lambda (script)
+ (chmod script #o555)
+ (install-file script bin))
+ scripts)
+ (substitute* (map (lambda (script)
+ (string-append bin "/" script))
+ scripts)
+ (("^java") (which "java"))
+ (("jar_deploy_dir=.*")
+ (string-append "jar_deploy_dir=" share "\n"))))
+ #t))
+ ;; FIXME: We do this after stripping jars because we don't want it to
+ ;; copy all these jars and strip them. We only want to install
+ ;; links. Arguably, this is a problem with the ant-build-system.
+ (add-after 'strip-jar-timestamps 'install-links
+ (lambda* (#:key outputs #:allow-other-keys)
+ (let* ((out (assoc-ref outputs "out"))
+ (share (string-append out "/share/java/"))
+ (lib (string-append share "/lib/")))
+ (for-each (lambda (jar)
+ (symlink (readlink jar)
+ (string-append lib (basename jar))))
+ (find-files "jar/lib" "\\.jar$")))
+ #t)))))
+ (inputs
+ `(("jdk" ,icedtea-8)
+ ("java-picard" ,java-picard-2.10.3)
+ ("java-log4j-1.2-api" ,java-log4j-1.2-api)
+ ("java-commons-math3" ,java-commons-math3)
+ ("java-commons-jexl2" ,java-commons-jexl-2)
+ ("java-commons-collections4" ,java-commons-collections4)
+ ("java-commons-lang2" ,java-commons-lang)
+ ("java-commons-io" ,java-commons-io)
+ ("java-snappy-1.0.3-rc3" ,java-snappy-1)
+ ("java-guava" ,java-guava)
+ ("java-la4j" ,java-la4j)
+ ("java-biojava-core" ,java-biojava-core-4.0)
+ ("java-biojava-alignment" ,java-biojava-alignment-4.0)
+ ("java-jdistlib" ,java-jdistlib)
+ ("java-simple-xml" ,java-simple-xml)
+ ("java-snakeyaml" ,java-snakeyaml)))
+ (native-inputs
+ `(("unzip" ,unzip)
+ ("java-testng" ,java-testng)))
+ (home-page "http://mccarrolllab.com/dropseq/")
+ (synopsis "Tools for Drop-seq analyses")
+ (description "Drop-seq is a technology to enable biologists to
+analyze RNA expression genome-wide in thousands of individual cells at
+once. This package provides tools to perform Drop-seq analyses.")
+ (license license:expat)))
+
+(define-public pigx-rnaseq
+ (package
+ (name "pigx-rnaseq")
+ (version "0.0.2")
+ (source (origin
+ (method url-fetch)
+ (uri (string-append "https://github.com/BIMSBbioinfo/pigx_rnaseq/"
+ "releases/download/v" version
+ "/pigx_rnaseq-" version ".tar.gz"))
+ (sha256
+ (base32
+ "168hx2ig3rarphx3l21ay9yyg8ipaakzixnrhpbdi0sknhyvrrk8"))))
+ (build-system gnu-build-system)
+ (arguments
+ `(#:parallel-tests? #f ; not supported
+ #:phases
+ (modify-phases %standard-phases
+ (add-after 'install 'wrap-executable
+ ;; Make sure the executable finds all R modules.
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let ((out (assoc-ref outputs "out")))
+ (wrap-program (string-append out "/bin/pigx-rnaseq")
+ `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))
+ `("PYTHONPATH" ":" = (,(getenv "PYTHONPATH")))))
+ #t)))))
+ (inputs
+ `(("snakemake" ,snakemake)
+ ("fastqc" ,fastqc)
+ ("multiqc" ,multiqc)
+ ("star" ,star)
+ ("trim-galore" ,trim-galore)
+ ("htseq" ,htseq)
+ ("samtools" ,samtools)
+ ("bedtools" ,bedtools)
+ ("r-minimal" ,r-minimal)
+ ("r-rmarkdown" ,r-rmarkdown)
+ ("r-ggplot2" ,r-ggplot2)
+ ("r-ggrepel" ,r-ggrepel)
+ ("r-gprofiler" ,r-gprofiler)
+ ("r-deseq2" ,r-deseq2)
+ ("r-dt" ,r-dt)
+ ("r-knitr" ,r-knitr)
+ ("r-pheatmap" ,r-pheatmap)
+ ("r-corrplot" ,r-corrplot)
+ ("r-reshape2" ,r-reshape2)
+ ("r-plotly" ,r-plotly)
+ ("r-scales" ,r-scales)
+ ("r-summarizedexperiment" ,r-summarizedexperiment)
+ ("r-crosstalk" ,r-crosstalk)
+ ("r-tximport" ,r-tximport)
+ ("r-rtracklayer" ,r-rtracklayer)
+ ("r-rjson" ,r-rjson)
+ ("salmon" ,salmon)
+ ("ghc-pandoc" ,ghc-pandoc)
+ ("ghc-pandoc-citeproc" ,ghc-pandoc-citeproc)
+ ("python-wrapper" ,python-wrapper)
+ ("python-pyyaml" ,python-pyyaml)))
+ (home-page "http://bioinformatics.mdc-berlin.de/pigx/")
+ (synopsis "Analysis pipeline for RNA sequencing experiments")
+ (description "PiGX RNAseq is an analysis pipeline for preprocessing and
+reporting for RNA sequencing experiments. It is easy to use and produces high
+quality reports. The inputs are reads files from the sequencing experiment,
+and a configuration file which describes the experiment. In addition to
+quality control of the experiment, the pipeline produces a differential
+expression report comparing samples in an easily configurable manner.")
+ (license license:gpl3+)))
+
+(define-public pigx-chipseq
+ (package
+ (name "pigx-chipseq")
+ (version "0.0.2")
+ (source (origin
+ (method url-fetch)
+ (uri (string-append "https://github.com/BIMSBbioinfo/pigx_chipseq/"
+ "releases/download/v" version
+ "/pigx_chipseq-" version ".tar.gz"))
+ (sha256
+ (base32
+ "1jliwhifnjgl9x0z730bzpxswi2s84fyg5y8cagbyzpw509452f5"))))
+ (build-system gnu-build-system)
+ (arguments
+ `(#:phases
+ (modify-phases %standard-phases
+ (add-after 'install 'wrap-executable
+ ;; Make sure the executable finds all R modules.
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let ((out (assoc-ref outputs "out")))
+ (wrap-program (string-append out "/bin/pigx-chipseq")
+ `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))
+ `("PYTHONPATH" ":" = (,(getenv "PYTHONPATH")))))
+ #t)))))
+ (inputs
+ `(("r-minimal" ,r-minimal)
+ ("r-argparser" ,r-argparser)
+ ("r-chipseq" ,r-chipseq)
+ ("r-data-table" ,r-data-table)
+ ("r-genomation" ,r-genomation)
+ ("r-genomicranges" ,r-genomicranges)
+ ("r-rtracklayer" ,r-rtracklayer)
+ ("r-rcas" ,r-rcas)
+ ("r-stringr" ,r-stringr)
+ ("r-jsonlite" ,r-jsonlite)
+ ("r-heatmaply" ,r-heatmaply)
+ ("r-ggplot2" ,r-ggplot2)
+ ("r-plotly" ,r-plotly)
+ ("python-wrapper" ,python-wrapper)
+ ("python-pyyaml" ,python-pyyaml)
+ ("snakemake" ,snakemake)
+ ("macs" ,macs)
+ ("multiqc" ,multiqc)
+ ("perl" ,perl)
+ ("ghc-pandoc" ,ghc-pandoc)
+ ("ghc-pandoc-citeproc" ,ghc-pandoc-citeproc)
+ ("fastqc" ,fastqc)
+ ("bowtie" ,bowtie)
+ ("idr" ,idr)
+ ("snakemake" ,snakemake)
+ ("samtools" ,samtools)
+ ("bedtools" ,bedtools)
+ ("kentutils" ,kentutils)))
+ (native-inputs
+ `(("python-pytest" ,python-pytest)))
+ (home-page "http://bioinformatics.mdc-berlin.de/pigx/")
+ (synopsis "Analysis pipeline for ChIP sequencing experiments")
+ (description "PiGX ChIPseq is an analysis pipeline for preprocessing, peak
+calling and reporting for ChIP sequencing experiments. It is easy to use and
+produces high quality reports. The inputs are reads files from the sequencing
+experiment, and a configuration file which describes the experiment. In
+addition to quality control of the experiment, the pipeline enables to set up
+multiple peak calling analysis and allows the generation of a UCSC track hub
+in an easily configurable manner.")
+ (license license:gpl3+)))
+
+(define-public pigx-bsseq
+ (package
+ (name "pigx-bsseq")
+ (version "0.0.5")
+ (source (origin
+ (method url-fetch)
+ (uri (string-append "https://github.com/BIMSBbioinfo/pigx_bsseq/"
+ "releases/download/v" version
+ "/pigx_bsseq-" version ".tar.gz"))
+ (sha256
+ (base32
+ "1h8ma99vi7hs83nafvjpq8jmaq9977j3n11c4zd95hai0cf7zxmp"))))
+ (build-system gnu-build-system)
+ (arguments
+ `(#:phases
+ (modify-phases %standard-phases
+ (add-before 'check 'set-timezone
+ ;; The readr package is picky about timezones.
+ (lambda* (#:key inputs #:allow-other-keys)
+ (setenv "TZ" "UTC+1")
+ (setenv "TZDIR"
+ (string-append (assoc-ref inputs "tzdata")
+ "/share/zoneinfo"))
+ #t))
+ (add-after 'install 'wrap-executable
+ ;; Make sure the executable finds all R modules.
+ (lambda* (#:key inputs outputs #:allow-other-keys)
+ (let ((out (assoc-ref outputs "out")))
+ (wrap-program (string-append out "/bin/pigx-bsseq")
+ `("R_LIBS_SITE" ":" = (,(getenv "R_LIBS_SITE")))
+ `("PYTHONPATH" ":" = (,(getenv "PYTHONPATH")))))
+ #t)))))
+ (native-inputs
+ `(("tzdata" ,tzdata)))
+ (inputs
+ `(("r-minimal" ,r-minimal)
+ ("r-annotationhub" ,r-annotationhub)
+ ("r-dt" ,r-dt)
+ ("r-genomation" ,r-genomation)
+ ("r-methylkit" ,r-methylkit)
+ ("r-rtracklayer" ,r-rtracklayer)
+ ("r-rmarkdown" ,r-rmarkdown)
+ ("r-bookdown" ,r-bookdown)
+ ("r-ggplot2" ,r-ggplot2)
+ ("r-ggbio" ,r-ggbio)
+ ("ghc-pandoc" ,ghc-pandoc)
+ ("ghc-pandoc-citeproc" ,ghc-pandoc-citeproc)
+ ("python-wrapper" ,python-wrapper)
+ ("python-pyyaml" ,python-pyyaml)
+ ("snakemake" ,snakemake)
+ ("bismark" ,bismark)
+ ("fastqc" ,fastqc)
+ ("bowtie" ,bowtie)
+ ("trim-galore" ,trim-galore)
+ ("cutadapt" ,cutadapt)
+ ("samtools" ,samtools)))
+ (home-page "http://bioinformatics.mdc-berlin.de/pigx/")
+ (synopsis "Bisulfite sequencing pipeline from fastq to methylation reports")
+ (description "PiGx BSseq is a data processing pipeline for raw fastq read
+data of bisulfite experiments; it produces reports on aggregate methylation
+and coverage and can be used to produce information on differential
+methylation and segmentation.")
+ (license license:gpl3+)))
generated by cgit v1.2.3 (git 2.46.2) at 2024-11-12 05:30:48 +0000